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Characterization of an enzymatic packed-bed microreactor: Experiments and modeling

Strnisa, F; Bajic, M; Panjan, P; Plazl, I; Sesay, AM; Znidarsic-Plazl, P; (2018) Characterization of an enzymatic packed-bed microreactor: Experiments and modeling. Chemical Engineering Journal , 350 pp. 541-550. 10.1016/j.cej.2018.05.028. Green open access

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Abstract

A micro packed-bed reactor (µPBR) based on two-parallel-plates configuration with immobilized Candida antarctica lipase B in the form of porous particles (Novozym® 435) was theoretically and experimentally characterized. A residence time distribution (RTD) within µPBRs comprising various random distributions of particles placed in one layer was computationally predicted by a mesoscopic lattice Boltzmann (LB) method. Numerical simulations were compared with measurements of RTD, obtained by stimulus-response experiment with a pulse input using glucose as a tracer, monitored by an electrochemical glucose oxidase microbiosensor integrated with the reactor. The model was validated by a good agreement between the experimental data and predictions of LB model at different conditions. The developed µPBR was scaled-up in length and width comprising either a single or two layers of Novozym® 435 particles and compared regarding the selected enzyme-catalyzed transesterification. A linear increase in the productivity with the increase in all dimensions of the µPBR between two-plates demonstrated very efficient and simple approach for the capacity rise. Further characterization of µPBRs of various sizes using the piezoresistive pressure sensor revealed very low pressure drops as compared to their conventional counterparts and thereby great applicability for production systems based on numbering-up approach.

Type: Article
Title: Characterization of an enzymatic packed-bed microreactor: Experiments and modeling
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.cej.2018.05.028
Publisher version: https://doi.org/10.1016/j.cej.2018.05.028
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Packed-bed microreactor, Biocatalysis, Transesterification, Residence time distribution, Lattice Boltzmann method, Glucose biosensor
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10120005
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