Chan, JM;
Dillard, JP;
(2016)
Neisseria gonorrhoeae Crippled Its Peptidoglycan Fragment Permease To Facilitate Toxic Peptidoglycan Monomer Release.
Journal of Bacteriology
, 198
(21)
pp. 3029-3040.
10.1128/JB.00437-16.
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Abstract
Neisseria gonorrhoeae (gonococci) and Neisseria meningitidis (meningococci) are human pathogens that cause gonorrhea and meningococcal meningitis respectively. Both N. gonorrhoeae and N. meningitidis release a number of small peptidoglycan (PG) fragments, including proinflammatory PG monomers, although N. meningitidis releases less of the PG monomers. The PG fragments released by N. gonorrhoeae and N. meningitidis are generated in the periplasm during cell wall remodeling, and a majority of these fragments are transported into the cytoplasm by an inner membrane permease, AmpG; however, a portion of the PG fragments are released into the extracellular environment through unknown mechanisms. We previously reported that expression of meningococcal ampG in N. gonorrhoeae reduced PG monomer release by gonococci. This finding suggested that the efficiency of AmpG-mediated PG fragment recycling regulates the amount of PG fragments released into the extracellular milieu. We determined that three AmpG residues near the C-terminal end of the protein modulate AmpG's efficiency. We also investigated the association between PG fragment recycling and release in two species of human-associated nonpathogenic Neisseria, N. sicca and N. mucosa. Both N. sicca and N. mucosa release lower levels of PG fragments and are more efficient at recycling PG fragments compared to N. gonorrhoeae. Our results suggest that N. gonorrhoeae has evolved to increase the amounts of toxic PG fragments released by reducing its PG recycling efficiency.
| Type: | Article |
|---|---|
| Title: | Neisseria gonorrhoeae Crippled Its Peptidoglycan Fragment Permease To Facilitate Toxic Peptidoglycan Monomer Release |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1128/JB.00437-16 |
| Publisher version: | http://dx.doi.org/10.1128/JB.00437-16 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | GONOCOCCAL GENETIC ISLAND, FALLOPIAN-TUBE MUCOSA, MENINGITIDIS GROUP-B, I-TASSER SERVER, SOLUBLE PEPTIDOGLYCAN, BORDETELLA-PERTUSSIS, TRACHEAL CYTOTOXIN, LYTIC TRANSGLYCOSYLASES, GROWING GONOCOCCI, PROTEIN-STRUCTURE |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10117224 |
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