UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Neisseria gonorrhoeae Crippled Its Peptidoglycan Fragment Permease To Facilitate Toxic Peptidoglycan Monomer Release

Chan, JM; Dillard, JP; (2016) Neisseria gonorrhoeae Crippled Its Peptidoglycan Fragment Permease To Facilitate Toxic Peptidoglycan Monomer Release. Journal of Bacteriology , 198 (21) pp. 3029-3040. 10.1128/JB.00437-16. Green open access

[thumbnail of Chan_Journal of Bacteriology-2016-Chan-JB.00437-16.full.pdf]
Preview
Text
Chan_Journal of Bacteriology-2016-Chan-JB.00437-16.full.pdf - Accepted Version

Download (1MB) | Preview

Abstract

Neisseria gonorrhoeae (gonococci) and Neisseria meningitidis (meningococci) are human pathogens that cause gonorrhea and meningococcal meningitis respectively. Both N. gonorrhoeae and N. meningitidis release a number of small peptidoglycan (PG) fragments, including proinflammatory PG monomers, although N. meningitidis releases less of the PG monomers. The PG fragments released by N. gonorrhoeae and N. meningitidis are generated in the periplasm during cell wall remodeling, and a majority of these fragments are transported into the cytoplasm by an inner membrane permease, AmpG; however, a portion of the PG fragments are released into the extracellular environment through unknown mechanisms. We previously reported that expression of meningococcal ampG in N. gonorrhoeae reduced PG monomer release by gonococci. This finding suggested that the efficiency of AmpG-mediated PG fragment recycling regulates the amount of PG fragments released into the extracellular milieu. We determined that three AmpG residues near the C-terminal end of the protein modulate AmpG's efficiency. We also investigated the association between PG fragment recycling and release in two species of human-associated nonpathogenic Neisseria, N. sicca and N. mucosa. Both N. sicca and N. mucosa release lower levels of PG fragments and are more efficient at recycling PG fragments compared to N. gonorrhoeae. Our results suggest that N. gonorrhoeae has evolved to increase the amounts of toxic PG fragments released by reducing its PG recycling efficiency.

Type: Article
Title: Neisseria gonorrhoeae Crippled Its Peptidoglycan Fragment Permease To Facilitate Toxic Peptidoglycan Monomer Release
Open access status: An open access version is available from UCL Discovery
DOI: 10.1128/JB.00437-16
Publisher version: http://dx.doi.org/10.1128/JB.00437-16
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: GONOCOCCAL GENETIC ISLAND, FALLOPIAN-TUBE MUCOSA, MENINGITIDIS GROUP-B, I-TASSER SERVER, SOLUBLE PEPTIDOGLYCAN, BORDETELLA-PERTUSSIS, TRACHEAL CYTOTOXIN, LYTIC TRANSGLYCOSYLASES, GROWING GONOCOCCI, PROTEIN-STRUCTURE
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/10117224
Downloads since deposit
47Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item