UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Process economics evaluation of cell-free synthesis for the commercial manufacture of antibody drug conjugates

Stamatis, C; Farid, SS; (2021) Process economics evaluation of cell-free synthesis for the commercial manufacture of antibody drug conjugates. Biotechnology Journal , 16 (4) , Article 2000238. 10.1002/biot.202000238. Green open access

[thumbnail of Stamatis Farid Process Eco CFS BiotJ20 AcceptedArticle.pdf]
Preview
Text
Stamatis Farid Process Eco CFS BiotJ20 AcceptedArticle.pdf - Accepted Version

Download (1MB) | Preview

Abstract

Continuous improvements of cell-free synthesis (CFS) systems have generated interest in adopting the technology for the manufacture of biologics. This paper provides an evaluation of the manufacturing cost-effectiveness of CFS for the commercial production of antibody-drug conjugates (ADCs). The evaluation was performed using an advanced techno-economic engine (TEE) built in Python. The TEE is programmed in an object-oriented environment capable of simulating a plethora of process flowsheets and predicting size and cost metrics for the process and the facility. A case study was formulated to compare the economics of whole bioprocesses based on either a CFS system or a mammalian cell system (CHO) for the manufacture of an ADC at a range of product demands. The analysis demonstrated the potential of CFS for the commercial manufacture of biologics and identified key cost drivers related to the system. The CFS system showed an approximately 80% increase in the cost of goods compared to CHO with a significant cost attributed to the in-house manufacture of the bacterial cell extract, necessary for the CFS reaction step in the process. A sensitivity and target analysis highlighted the need for further process improvements especially in the titre for the CFS process to become more competitive against well-established systems. This article is protected by copyright. All rights reserved.

Type: Article
Title: Process economics evaluation of cell-free synthesis for the commercial manufacture of antibody drug conjugates
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/biot.202000238
Publisher version: http://dx.doi.org/10.1002/biot.202000238
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Antibody-drug conjugates, cell-free synthesis, manufacture, process economics, techno-economic analysis
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10116483
Downloads since deposit
312Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item