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Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy

Surowka, AD; Gianoncelli, A; Birarda, G; Sala, S; Cefarin, N; Matruglio, A; Szczerbowska-Boruchowska, M; ... Vaccari, L; + view all (2020) Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy. Journal of Synchrotron Radiation , 27 pp. 1218-1226. 10.1107/S1600577520010103. Green open access

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Abstract

In order to push the spatial resolution limits to the nanoscale, synchrotron-based soft X-ray microscopy (XRM) experiments require higher radiation doses to be delivered to materials. Nevertheless, the associated radiation damage impacts on the integrity of delicate biological samples. Herein, the extent of soft X-ray radiation damage in popular thin freeze-dried brain tissue samples mounted onto Si3N4 membranes, as highlighted by Fourier transform infrared microscopy (FTIR), is reported. The freeze-dried tissue samples were found to be affected by general degradation of the vibrational architecture, though these effects were weaker than those observed in paraffin-embedded and hydrated systems reported in the literature. In addition, weak, reversible and specific features of the tissue–Si3N4 interaction could be identified for the first time upon routine soft X-ray exposures, further highlighting the complex interplay between the biological sample, its preparation protocol and X-ray probe.

Type: Article
Title: Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy
Open access status: An open access version is available from UCL Discovery
DOI: 10.1107/S1600577520010103
Publisher version: http://dx.doi.org/10.1107/S1600577520010103
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: FTIR microscopy, X-ray microscopy, freeze-dried brain tissue, FLUORESCENCE MICROSCOPY, DOSE LIMITS, TRANSMISSION, MECHANISMS, BEAMLINE
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10115401
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