UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Motility and chemotaxis studies in Helicobacter pylori

Foynes, Susan; (1999) Motility and chemotaxis studies in Helicobacter pylori. Doctoral thesis (Ph.D), UCL (University College London). Green open access

[thumbnail of Motility_and_chemotaxis_studie.pdf] Text

Download (15MB)


H. pylori is one of the most common causes of chronic bacterial infection in man and its pathogenic role in the development of gastric and duodenal ulcers and gastric cancer is well documented. Chemotaxis is thought to be important in enabling H. pylori to reach the surface mucus layer in the stomach of infected subjects. Little is known about the mechanism of the chemotactic response or of its role played in the motility and virulence of H. pylori. Therefore the first step was to amplify and clone cheY and cheA homologues from H. pylori. Both CheY1 and CheAY had high identity to those found in other bacterial species. H. pylori N6 cheY1, cheAY, cheY, cheAY/Y1 and H. pylori SS1 cheY1 and cheAY isogenic mutants were constructed. The pattern of the chemotactic response was studied using swarm plates, capillary tube assays and computerised motility analysis. H. pylori CheAY seems likely to interact with the flagellar motor to bring about motor switching, whilst the CheY1 homologue acts to terminate the response. Mucin and urea were confirmed as chemoattractants and the results of the study were combined to produced a model of chemotaxis which differs from the E. coli paradigm in many respects. Finally the chemotaxis mutants were unable to colonise both the gnotobiotic piglet and mouse animal models confirming chemotaxis as a requirement for pathogenicity in H. pylori.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: Motility and chemotaxis studies in Helicobacter pylori
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: Biological sciences; Health and environmental sciences; Helicobacter pylori
URI: https://discovery.ucl.ac.uk/id/eprint/10104747
Downloads since deposit
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item