Wang, Yan;
(1995)
Agalactosyl IgG: Its clinical associations, biological properties, and relationship to infection.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
In this study, the immunoassay for measuring the level of agalactosyl IgG has been developed into a more simple and efficient version. Using this method, an increase in serum level of agalactosyl IgG has been demonstrated in patients with rheumatoid arthritis, sarcoidosis and Takayasu's arteritis. The second part of the study addressed the regulation of agalactosyl IgG by interleukin-6 (IL-6). A significant correlation has been shown between serum IL-6 titres and percentage agalactosyl IgG in patients with sarcoidosis. The serum level of agalactosyl IgG was also correlated with the IL-6 titres in peritoneal exudate fluid in pristane-injected mice. Moreover, the injection of recombinant IL-6 into normal mice increased their serum level of agalactosyl IgG. However, in patients with rheumatoid arthritis, no significant correlation between agalactosyl IgG and IL-6 was found. Furthermore, an inhibitor of lL-6 previously found in sera of sarcoidosis patients using the B9 cell line was shown not to be specific for IL-6, and inhibited the growth of other cell types. In the third part of the study, a reduction was demonstrated in binding of the agalactosyl IgG3 to human FcỿRII isoforms compared to the galactosyl IgG3. This suggested the possibility that negative feedback on B cells via Fc receptors might be defective, but in vivo experiment with agalactosyl IgG showed that such feedback was normal. The importance of these findings was discussed in relation to autoimmune diseases and abnormal humoral immune response evoked by infectious agents.
Type: | Thesis (Doctoral) |
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Qualification: | Ph.D |
Title: | Agalactosyl IgG: Its clinical associations, biological properties, and relationship to infection. |
Open access status: | An open access version is available from UCL Discovery |
Language: | English |
Additional information: | Thesis digitised by ProQuest. |
Keywords: | Biological sciences; Agalactosyl IgG; Biological properties; Clinical associations; Infection |
URI: | https://discovery.ucl.ac.uk/id/eprint/10104453 |
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