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The effect of phosphate based glasses on the formation and viability of oral bacterial biofilms

Mulligan, April Miranda; (2003) The effect of phosphate based glasses on the formation and viability of oral bacterial biofilms. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

This study considered the antibacterial activity of a series of soluble phosphate-based glasses (based on the Na2O-CaO-P2O5 glass system) doped with increasing amounts of copper or silver against oral bacterial biofilms. Initially, a variety of phosphate-based glass compositions were produced. The dissolution rate of these glasses was determined, and the information obtained was used to decide which glass compositions would be investigated in future experiments for their antibacterial properties. Selected glass compositions were investigated for their antibacterial activity against Streptococcus sanguis biofilms and oral microcosm biofilms. These biofilms were produced on phosphate-based glass discs using a Constant Depth Film Fermenter (CDFF), which allows the conditions found in the oral cavity to be closely mimicked. Following disc removal from the CDFF, various analytical procedures were carried out. Under conditions designed to mimic the supragingival environment of the oral cavity, fewer viable cells of Streptococcus sanguis were detected on both copper and silver-containing glass discs than on control discs, during the initial stages of the experiments, the greatest reduction occurring on the silver-containing glasses. An increase in viable cell number was observed as the experiments continued. Under the same conditions, copper-containing glasses failed to reduce the viability of microcosm biofilms. Viable cell number was initially reduced on the silver-containing glasses, but by the end of the experiments the viability of microcosm biofilms was significantly similar to those observed on the controls. Attempts to determine the efficacy of silver-containing glasses at reducing the viability of microcosm biofilms, under conditions designed to mimic the subgingival environment of the oral cavity, were subsequently made. Viable cells were not detected on any type of disc, including the control discs. Various reasons for this were postulated. In conclusion, the glasses investigated exhibited some ability to reduce the viability of oral bacterial biofilms, silver glasses proving more effective, although they did not manage to maintain viable counts at the low levels initially observed.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: The effect of phosphate based glasses on the formation and viability of oral bacterial biofilms
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
Keywords: Applied sciences; Phosphate-based glasses
URI: https://discovery.ucl.ac.uk/id/eprint/10102016
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