Jayasinghe, SN;
(2020)
Reimagining Flow Cytometric Cell Sorting.
Advanced Biosystems
, Article e2000019. 10.1002/adbi.202000019.
(In press).
Preview |
Text
adbi.202000019.pdf - Published Version Download (1MB) | Preview |
Abstract
In this review, a brief history of this unrivaled technology, flow cytometry, is provided, highlighting its past and present advances, with particular focus on "flow cell" technologies. Flow cytometry has truly revolutionized high-throughput single cell analysis, which has tremendous implications, from laboratory to the clinic. This technology embodies what is truly referred to as cross fertile research, merging the physical with the life sciences. This review introduces the recent notable advancements in flow cell technology. This advancement sees the complete removal of liquid sheath flow, which has advanced the technology with the possibility of both the reduction in its foot print, while also simplifying the flow cells explored in cytometry. Interestingly, the novel sheathless flow cell technology demonstrated herein has the flexibility for handling both heterogeneous cell populations and whole organisms, thus demonstrating a versatile flow cell technology for both flow cytometry and fluorescent-activated cell sorting.
| Type: | Article |
|---|---|
| Title: | Reimagining Flow Cytometric Cell Sorting |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/adbi.202000019 |
| Publisher version: | http://dx.doi.org/10.1002/adbi.202000019 |
| Language: | English |
| Additional information: | © 2020 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | FACS, aerodynamically assisted biojets, cell sorting, flow cells, sheathless flow cytometry, single cell diagnostics |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10100203 |
Archive Staff Only
 |
View Item |
