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Insights into the design of spray systems for cell therapies for retinal disease using computational modelling

Nweze, M; Baker, T; Limb, GA; Shipley, RJ; (2020) Insights into the design of spray systems for cell therapies for retinal disease using computational modelling. Mathematical Biosciences and Engineering , 17 (3) pp. 2741-2759. 10.3934/mbe.2020150. Green open access

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Abstract

Chronic eye diseases are the main cause of vision loss among adults. Among these, retinal degenerative diseases affect millions of people globally, causing permanent loss of cells and organ dysfunction. Despite recent progress in developing stem cell therapies for retinal diseases, methods for delivery remain an area of intense research. Aerosol technology is a promising technique with the potential to spray cells evenly and directly across the retinal surface, promoting cell attachment and survival. Here we implement mathematical modelling of the spraying process to develop organ-specific spraying parameters in this therapeutic scenario. Firstly, we characterise the rheological parameters for a typical hydrogel used for spraying cells. These parameters are then integrated into a 3D computational model of an adult human eye under realistic surgical conditions. Simulation results provide quantitative relationships between the volume flow rate of the cell-laden hydrogel, external pressure needed for aerosolization, angle of the spraying, and properties of the cell delivery. An experimental assessment is also carried out to explore the impact of spraying under the regimes identified by the computational model on cell viability. This is the first stage towards using computational models to inform the design of spray systems to deliver cell therapies onto the human retina.

Type: Article
Title: Insights into the design of spray systems for cell therapies for retinal disease using computational modelling
Open access status: An open access version is available from UCL Discovery
DOI: 10.3934/mbe.2020150
Publisher version: https://doi.org/10.3934/mbe.2020150
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Science & Technology, Life Sciences & Biomedicine, Mathematical & Computational Biology, computational modelling, retina, cell-based therapy, cell spraying, cell delivery, DEMUCOSALIZED COLONIC SEGMENTS, SMOOTH-MUSCLE-CELLS, AEROSOL TRANSFER, FIBRIN GLUE, IN-VIVO, DELIVERY, REPAIR
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10094589
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