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A calibrated optogenetic toolbox of stable zebrafish opsin lines

Antinucci, P; Dumitrescu, A; Deleuze, C; Morley, HJ; Leung, K; Hagley, T; Kubo, F; ... Wyart, C; + view all (2020) A calibrated optogenetic toolbox of stable zebrafish opsin lines. eLife , 9 , Article e54937. 10.7554/eLife.54937. (In press). Green open access

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Abstract

Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity with subcellular resolution and millisecond precision. Achieving reliable and interpretable in vivo optogenetic manipulations requires reproducible actuator expression and calibration of photocurrents in target neurons. Here, we developed nine transgenic zebrafish lines for stable opsin expression and calibrated their efficacy in vivo. We first used high-throughput behavioural assays to compare opsin ability to elicit or silence neural activity. Next, we performed in vivo whole-cell electrophysiological recordings to quantify the amplitude and kinetics of photocurrents and test opsin ability to precisely control spiking. We observed substantial variation in efficacy, associated with differences in both opsin expression level and photocurrent characteristics, and identified conditions for optimal use of the most efficient opsins. Overall, our calibrated optogenetic toolkit will facilitate the design of controlled optogenetic circuit manipulations.

Type: Article
Title: A calibrated optogenetic toolbox of stable zebrafish opsin lines
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.54937
Publisher version: https://doi.org/10.7554/eLife.54937
Language: English
Additional information: © 2020, Antinucci et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
Keywords: neuroscience, zebrafish
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/10094379
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