Tosheva, KL;
Yuan, Y;
Pereira, PM;
Culley, S;
Henriques, R;
(2020)
Between life and death: strategies to reduce phototoxicity in super-resolution microscopy.
Journal of Physics D: Applied Physics
10.1088/1361-6463/ab6b95.
(In press).
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Abstract
Super-Resolution Microscopy enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we overview new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.
| Type: | Article |
|---|---|
| Title: | Between life and death: strategies to reduce phototoxicity in super-resolution microscopy |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1088/1361-6463/ab6b95 |
| Publisher version: | https://doi.org/10.1088/1361-6463/ab6b95 |
| Language: | English |
| Additional information: | As the Version of Record of this article is going to be/has been published on a gold open access basis under a CC BY 3.0 licence, this Accepted Manuscript is available for reuse under a CC BY 3.0 licence immediately. Although reasonable endeavours have been taken to obtain all necessary permissions from third parties to include their copyrighted content within this article, their full citation and copyright line may not be present in this Accepted Manuscript version. Before using any content from this article, please refer to the Version of Record on IOPscience once published for full citation and copyright details, as permission may be required. All third party content is fully copyright protected, and is not published on a gold open access basis under a CC BY licence, unless that is specifically stated in the figure caption in the Version of Record. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10090447 |
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