UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Generation of a neutralizing antibody against RD114-pseudotyped viral vectors

Mekkaoui, L; Ferrari, M; Mattiuzzo, G; Ma, B; Nannini, F; Onuoha, S; Kotsopoulou, E; ... Pule, M; + view all (2019) Generation of a neutralizing antibody against RD114-pseudotyped viral vectors. Journal of General Virology 10.1099/jgv.0.001309. (In press). Green open access

[thumbnail of Takeuchi_Generation of a neutralizing antibody against RD114-pseudotyped viral vectors_AAM.pdf]
Preview
Text
Takeuchi_Generation of a neutralizing antibody against RD114-pseudotyped viral vectors_AAM.pdf - Accepted Version

Download (1MB) | Preview

Abstract

The feline endogenous RD114 glycoprotein has proved to be an attractive envelope to pseudotype both retroviral and lentiviral vectors. As a surface protein, its detection on packaging cells as well as viral particles would be useful in different fields of its use. To address this, we generated a monoclonal antibody against RD114 by immunization of rats, termed 22F10. Once seroconversion was confirmed, purified 22F10 was cloned into murine Fc and characterized with a binding affinity of 10nM. The antibody was used to detect RD114 and its variant envelopes on different stable viral packaging cell lines (FLYRD18 and WinPac-RD). 22F10 was also shown to prevent the infections of different strains of RD-pseudotyped vectors but not related envelope glycoproteins by blocking cell surface receptor binding. We are the first to report the neutralization of viral particles by a monoclonal αRD114 antibody.

Type: Article
Title: Generation of a neutralizing antibody against RD114-pseudotyped viral vectors
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1099/jgv.0.001309
Publisher version: https://doi.org/10.1099/jgv.0.001309
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: RD114, detection, monoclonal antibody, neutralization
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/10088300
Downloads since deposit
411Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item