Mekkaoui, L;
Ferrari, M;
Mattiuzzo, G;
Ma, B;
Nannini, F;
Onuoha, S;
Kotsopoulou, E;
... Pule, M; + view all
(2019)
Generation of a neutralizing antibody against RD114-pseudotyped viral vectors.
Journal of General Virology
10.1099/jgv.0.001309.
(In press).
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Takeuchi_Generation of a neutralizing antibody against RD114-pseudotyped viral vectors_AAM.pdf - Accepted Version Download (1MB) | Preview |
Abstract
The feline endogenous RD114 glycoprotein has proved to be an attractive envelope to pseudotype both retroviral and lentiviral vectors. As a surface protein, its detection on packaging cells as well as viral particles would be useful in different fields of its use. To address this, we generated a monoclonal antibody against RD114 by immunization of rats, termed 22F10. Once seroconversion was confirmed, purified 22F10 was cloned into murine Fc and characterized with a binding affinity of 10nM. The antibody was used to detect RD114 and its variant envelopes on different stable viral packaging cell lines (FLYRD18 and WinPac-RD). 22F10 was also shown to prevent the infections of different strains of RD-pseudotyped vectors but not related envelope glycoproteins by blocking cell surface receptor binding. We are the first to report the neutralization of viral particles by a monoclonal αRD114 antibody.
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