Shaw, HA;
Khodadoost, L;
Preston, MD;
Corver, J;
Mullany, P;
Wren, BW;
(2019)
Clostridium difficile clade 3 (RT023) have a modified cell surface and contain a large transposable island with novel cargo.
Scientific Reports
, 9
, Article 15330. 10.1038/s41598-019-51628-5.
Preview |
Text
Mullany_Clostridium difficile clade 3 (RT023) have a modified cell surface and contain a large transposable island with novel cargo_VoR.pdf - Published Version Download (3MB) | Preview |
Abstract
The major global pathogen Clostridium difcile (recently renamed Clostridioides difcile) has large genetic diversity including multiple mobile genetic elements. In this study, whole genome sequencing of 86 strains from the poorly characterised clade 3, predominantly PCR ribotype (RT)023, of C. difcile revealed distinctive surface architecture characteristics and a large mobile genetic island. These strains have a unique sortase substrate phenotype compared with well-characterised strains of C. difcile, and loss of the phage protection protein CwpV. A large genetic insertion (023_CTnT) comprised of three smaller elements (023_CTn1-3) is present in 80/86 strains analysed in this study, with genes common among other bacterial strains in the gut microbiome. Novel cargo regions of 023_CTnT include genes encoding a sortase, putative sortase substrates, lantibiotic ABC transporters and a putative siderophore biosynthetic cluster. We demonstrate the excision of 023_CTnT and sub-elements 023_CTn2 and 023_CTn3 from the genome of RT023 reference strain CD305 and the transfer of 023_CTn3 to a nontoxigenic C. difcile strain, which may have implications for the use of non-toxigenic C. difcile strains as live attenuated vaccines. Finally, we show that the genes within the island are expressed in a regulated manner in C. difcile RT023 strains conferring a distinct “niche adaptation”.
| Type: | Article |
|---|---|
| Title: | Clostridium difficile clade 3 (RT023) have a modified cell surface and contain a large transposable island with novel cargo |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41598-019-51628-5 |
| Publisher version: | https://doi.org/10.1038/s41598-019-51628-5 |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10085813 |
Archive Staff Only
 |
View Item |
