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Isolation and Characterisation of Human Adipose-Derived Stem Cells

Boyd, A; Wilson, A; Chee, M; (2019) Isolation and Characterisation of Human Adipose-Derived Stem Cells. In: Boyd, A, (ed.) Immunological Tolerance. (pp. 3-13). Humana Press: New York, NY, USA. Green open access

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Abstract

Recently, adipose-derived stem cells (ASCs), obtained from fresh human lipoaspirate, have shown promise as immunomodulatory agents having demonstrated immunosuppressive functionality both in vitro and in vivo. A number of researchers have described the isolation of ASCs through the enzymatic digestion of fat samples, followed by a number of purification steps, involving centrifugation and filtration. Here, we utilize a standard isolation technique, with the added purification of putative ASCs by fluorescence activated cell sorting (FACS). ASCs are an extremely valuable resource in clinical applications, including reconstruction, regeneration, and investigations into immune activity. This method could be used to isolate and purify ASCs for such downstream applications.

Type: Book chapter
Title: Isolation and Characterisation of Human Adipose-Derived Stem Cells
ISBN-13: 978-1-4939-8936-2
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-4939-8938-6_1
Publisher version: https://link.springer.com/protocol/10.1007%2F978-1...
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Adipose-derived stem cells, Adipose stem cells, ASCs, Isolation, Characterization, Immunosuppression, Immunomodulation, Stromal vascular fraction, Collagenase, Lipotransfer, Lipoaspirate
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/10077186
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