UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Type of RNA Packed in VLPs Impacts IgG Class Switching-Implications for an Influenza Vaccine Design

C Gomes, A; Roesti, ES; El-Turabi, A; Bachmann, MF; (2019) Type of RNA Packed in VLPs Impacts IgG Class Switching-Implications for an Influenza Vaccine Design. Vaccines , 7 (2) , Article 47. 10.3390/vaccines7020047. Green open access

[thumbnail of Cruz Gomes_Type of RNA Packed in VLPs Impacts IgG Class Switching-Implications for an Influenza Vaccine Design_VoR.pdf]
Preview
Text
Cruz Gomes_Type of RNA Packed in VLPs Impacts IgG Class Switching-Implications for an Influenza Vaccine Design_VoR.pdf - Published Version

Download (2MB) | Preview

Abstract

Nucleic acid packed within virus-like particles (VLPs) is shown to shape the immune response and to induce stronger B cell responses in different immunisation models. Here, using a VLP displaying the highly conserved extracellular domain of the M2 protein (M2e) from the influenza viruses as an antigen, we demonstrate that the type of RNA packaged into VLPs can alter the quality of the induced humoral response. By comparing prokaryotic RNA (pRNA), eukaryotic RNA (eRNA) and transfer RNA (tRNA), we find that pRNA induces the most protective IgG subclasses using a murine influenza model. We provide evidence that this process is predominantly dependent on endosomal Toll-like receptor (TLR7), and rule out a role for cytoplasmic mitochondrial antiviral signalling protein (MAVS) and its upstream retinoic acid-inducible gene-I-like receptors (RIG-I). Our findings provide considerations for the rational design of VLP-based vaccines and the immunomodulation exerted by TLR7 ligands packaged within the particles. Based on this work, we conclude that VLPs packing prokaryotic RNA must be preferred whenever a response dominated by IgG2 is desired, while eukaryotic RNA should be employed in order to induce a response dominated by IgG1.

Type: Article
Title: Type of RNA Packed in VLPs Impacts IgG Class Switching-Implications for an Influenza Vaccine Design
Location: Switzerland
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/vaccines7020047
Publisher version: https://doi.org/10.3390/vaccines7020047
Language: English
Additional information: © 2019 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Keywords: IgG, RNA, TLR7, VLPs
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/10075944
Downloads since deposit
75Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item