Cuff, S;
Lewis, RD;
Chinje, E;
Jaffar, M;
Knox, R;
Weeks, I;
(2018)
An improved cell-permeable fluorogenic substrate as the basis for a highly sensitive test for NAD(P)H quinone oxidoreductase 1 (NQO1) in living cells.
Free Radical Biology and Medicine
, 116
pp. 141-148.
10.1016/j.freeradbiomed.2018.01.009.
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Abstract
NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoenzyme upregulated in response to oxidative stress and in some cancers. Its upregulation by compounds has been used as an indicator of their potential anti-cancer properties. In this study we have designed, produced and tested a fluorogenic coumarin conjugate which selectively releases highly fluorescent 4-methylumbelliferone (4-MU) in the presence of NQO1. It was found that measuring 4-MU release rapidly and specifically quantitated NQO1 levels in vitro and in live cells. Both the substrate and its products freely perfused through cell membranes and were non-toxic. The substrate was very specific with low background, and the assay itself could be done in less than 10 minutes. This is the first assay to allow the quantitation of NQO1 in live cells which can then be retained for further experiments.
| Type: | Article |
|---|---|
| Title: | An improved cell-permeable fluorogenic substrate as the basis for a highly sensitive test for NAD(P)H quinone oxidoreductase 1 (NQO1) in living cells |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.freeradbiomed.2018.01.009 |
| Publisher version: | https://doi.org/10.1016/j.freeradbiomed.2018.01.00... |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | High throughput screening, NQO1, Cancer, Natural products, Analytical methods |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10075134 |
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