Clare, DK;
Siebert, CA;
Hecksel, C;
Hagen, C;
Mordhorst, V;
Grange, M;
Ashton, AW;
... Zhang, P; + view all
(2017)
Electron Bio-Imaging Centre (eBIC): the UK national research facility for biological electron microscopy.
Acta Crystallographica Section D - Structural Biology
, 73
pp. 488-495.
10.1107/S2059798317007756.
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Abstract
The recent resolution revolution in cryo-EM has led to a massive increase in demand for both time on high-end cryo-electron microscopes and access to cryo-electron microscopy expertise. In anticipation of this demand, eBIC was set up at Diamond Light Source in collaboration with Birkbeck College London and the University of Oxford, and funded by the Wellcome Trust, the UK Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC) to provide access to high-end equipment through peer review. eBIC is currently in its start-up phase and began by offering time on a single FEI Titan Krios microscope equipped with the latest generation of direct electron detectors from two manufacturers. Here, the current status and modes of access for potential users of eBIC are outlined. In the first year of operation, 222 d of microscope time were delivered to external research groups, with 95 visits in total, of which 53 were from unique groups. The data collected have generated multiple high- to intermediate-resolution structures (2.8–8 Å), ten of which have been published. A second Krios microscope is now in operation, with two more due to come online in 2017. In the next phase of growth of eBIC, in addition to more microscope time, new data-collection strategies and sample-preparation techniques will be made available to external user groups. Finally, all raw data are archived, and a metadata catalogue and automated pipelines for data analysis are being developed.
| Type: | Article |
|---|---|
| Title: | Electron Bio-Imaging Centre (eBIC): the UK national research facility for biological electron microscopy |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1107/S2059798317007756 |
| Publisher version: | https://doi.org/10.1107/S2059798317007756 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Biochemistry & Molecular Biology, Biophysics, Crystallography, Electron Bio-Imaging Centre, eBIC, cryo-EM, cryo-ET, user facilities, CRYO-EM STRUCTURE, NEAR-ATOMIC-RESOLUTION, CRYOELECTRON MICROSCOPY, STRUCTURAL BIOLOGY, VITREOUS SECTIONS, MOLECULAR-BASIS, TOMOGRAPHY, COMPLEX, CRYOMICROSCOPY, REVEALS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10073369 |
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