UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

The absence or presence of a lytic coliphage affects the response of Escherichia coli to heat, chlorine, or UV exposure

Ameh, EM; Tyrrel, S; Harris, J; Ignatiou, A; Orlova, E; Nocker, A; (2018) The absence or presence of a lytic coliphage affects the response of Escherichia coli to heat, chlorine, or UV exposure. Folia Microbiologica , 63 (5) pp. 599-606. 10.1007/s12223-018-0600-9. Green open access

[thumbnail of The_absence_or_presence_of_a_lytic_coliphage-2018.pdf]
Preview
Text
The_absence_or_presence_of_a_lytic_coliphage-2018.pdf - Accepted Version

Download (987kB) | Preview

Abstract

Disinfection aims at maximal inactivation of target organisms and the sustainable suppression of their regrowth. Whereas many disinfection efforts achieve efficient inactivation when the effect is measured directly after treatment, there are questions about the sustainability of this effect. One aspect is that the treated bacteria might recover and regain the ability to grow. In an environmental context, another question is how amenable surviving bacteria are to predation by omnipresent bacteriophages. Provisional data suggested that bacteria when subjected to sublethal heat stress might develop a phage-resistant phenotype. The result made us wonder about the susceptibility to phage-mediated lysis for bacteria exposed to a gradient of chlorine and UV-LED disinfection strengths. Whereas bacteria exposed to low sublethal chlorine doses still underwent phage-mediated lysis, the critical chlorine Ct of 0.5 mg min/L eliminated this susceptibility and induced phage resistance in the cells that survived treatment. In the case of UV, even the smallest tested dose of 2.8 mJ/cm2 abolished phage lysis leading to direct regrowth. Results suggest that bacteria surviving disinfection might have higher environmental survival chances directly after treatment compared to non-treated cells. A reason could possibly lie in their compromised metabolism that is essential for phage replication.

Type: Article
Title: The absence or presence of a lytic coliphage affects the response of Escherichia coli to heat, chlorine, or UV exposure
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s12223-018-0600-9
Publisher version: https://doi.org/10.1007/s12223-018-0600-9
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Bacteriolysis, Chlorine, Coliphages, Colony Count, Microbial, Disinfection, Escherichia coli, Flow Cytometry, Hot Temperature, Microbial Viability, Stress, Physiological, Ultraviolet Rays
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10073363
Downloads since deposit
70Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item