UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Measuring CTLA-4-Dependent Suppressive Function in Regulatory T Cells

Hou, TZ; Qureshi, OS; Sansom, DM; (2019) Measuring CTLA-4-Dependent Suppressive Function in Regulatory T Cells. Immunological Tolerance. Methods in Molecular Biology , 1899 pp. 87-101. 10.1007/978-1-4939-8938-6_7. Green open access

[thumbnail of Hou final submitted non format.pdf]
Preview
Text
Hou final submitted non format.pdf - Accepted Version

Download (677kB) | Preview

Abstract

Regulatory T cells (Treg) have a central role in controlling the activation of self-reactive T cells and maintaining peripheral tolerance in our body. Many effector mechanisms for Treg function have been described including a role for the protein CTLA-4 which is constitutively expressed by these cells. Despite its importance, there is currently little consensus in the methods and protocols for studying CTLA-4 function, which is partially due to debate over CTLA-4 function itself. In this chapter, we outline protocols used in our lab to study CTLA-4 function, which have been generated based on the observation that CTLA-4 acts to physically remove and degrade its ligands expressed by antigen presenting cells. Accordingly, we provide protocols for isolation of human monocytes and their differentiation into dendritic cells (DC), purification of conventional and regulatory T-cell populations, and the assembly of CTLA-4-dependent Treg suppression assays. We hope that this will offer a reliable platform for dissecting the biology of CTLA-4 on Treg and for testing reagents aimed at modulating CTLA-4 function. Such assays are increasingly vital for the study of immune function in both healthy individuals and patients with a variety of autoimmune and immune dysregulation syndromes.

Type: Article
Title: Measuring CTLA-4-Dependent Suppressive Function in Regulatory T Cells
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-4939-8938-6_7
Publisher version: http://doi.org/10.1007/978-1-4939-8938-6_7
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: CD28, CD80, CD86, CTLA-4, Conventional T cells, Costimulation, Dendritic cell, Regulatory T cells, Suppression, Transendocytosis
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/10072683
Downloads since deposit
347Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item