Parkes, M;
Bennett, A;
Fielding, H;
(2019)
A photoelectron imaging and quantum chemistry study of the deprotonated cyan fluorescent protein chromophore anion.
Molecular Physics
, 117
(21)
pp. 3027-2035.
10.1080/00268976.2019.1603410.
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Abstract
Cyan fluorescent protein (CFP), a blue-shifted analogue of green fluorescent protein, is used widely as a fluorescence resonance energy transfer donor in live cell fluorescence imaging. Here, we use a combination of anion photoelectron spectroscopy experiments and quantum chemistry calculations to probe the electronic structure of the CFP chromophore in its deprotonated anionic form. The vertical detachment energy measured as the maximum in the photoelectron spectrum is 2.75±0.02 eV, which is an improvement on our earlier measurement. We see evidence for competing internal conversion following resonant excitation of electronically excited states of the anion lying in the detachment continuum. We find that the first electronically excited valence state of the anion lies very close to the detachment threshold, supporting the conclusions of gas-phase photodestruction action spectroscopy measurements, and that the second electronically excited valence state lies around 1.25 eV above the detachment threshold. These electronic states have shape resonance character and mixed excited shape and Feshbach resonance character, respectively.
| Type: | Article |
|---|---|
| Title: | A photoelectron imaging and quantum chemistry study of the deprotonated cyan fluorescent protein chromophore anion |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1080/00268976.2019.1603410 |
| Publisher version: | https://doi.org/10.1080/00268976.2019.1603410 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Anion photoelectron spectroscopy, autodetachment, fluorescent proteins, photochemistry, photophysics |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10072481 |
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