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Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle

Capel, A; Rimington, R; Fleming, J; Player, D; Baker, L; Turner, M; Jones, J; ... Lewis, M; + view all (2019) Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle. Frontiers in Bioengineering and Biotechnology , 7 , Article 20. 10.3389/fbioe.2019.00020. Green open access

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Abstract

Tissue engineered skeletal muscle allows investigation of the cellular and molecular mechanisms that regulate skeletal muscle pathology. The fabricated model must resemble characteristics of in vivo tissue and incorporate cost-effective and high content primary human tissue. Current models are limited by low throughput due to the complexities associated with recruiting tissue donors, donor specific variations, as well as cellular senescence associated with passaging. This research presents a method using fused deposition modeling (FDM) and laser sintering (LS) 3D printing to generate reproducible and scalable tissue engineered primary human muscle, possessing aligned mature myotubes reminiscent of in vivo tissue. Many existing models are bespoke causing variability when translated between laboratories. To this end, a scalable model has been developed (25–500 μL construct volumes) allowing fabrication of mature primary human skeletal muscle. This research provides a strategy to overcome limited biopsy cell numbers, enabling high throughput screening of functional human tissue.

Type: Article
Title: Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fbioe.2019.00020
Publisher version: http://dx.doi.org/10.3389/fbioe.2019.00020
Language: English
Additional information: © 2019 Capel, Rimington, Fleming, Player, Baker, Turner, Jones, Martin, Ferguson, Mudera and Lewis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/).
Keywords: primary skeletal muscle, tissue engineering, 3D printing, skeletal muscle physiology, bioengineering
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: https://discovery.ucl.ac.uk/id/eprint/10068693
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