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Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa

Salih, O; He, S; Planamente, S; Stach, L; MacDonald, JT; Manoli, E; Scheres, SHW; ... Freemont, PS; + view all (2018) Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa. Structure , 26 (2) pp. 329-336. 10.1016/j.str.2017.12.005. Green open access

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Abstract

Pseudomonas aeruginosa has three type VI secretion systems (T6SSs), H1-, H2-, and H3-T6SS, each belonging to a distinct group. The two T6SS components, TssB/VipA and TssC/VipB, assemble to form tubules that conserve structural/functional homology with tail sheaths of contractile bacteriophages and pyocins. Here, we used cryoelectron microscopy to solve the structure of the H1-T6SS P. aeruginosa TssB1C1 sheath at 3.3 Å resolution. Our structure allowed us to resolve some features of the T6SS sheath that were not resolved in the Vibrio cholerae VipAB and Francisella tularensis IglAB structures. Comparison with sheath structures from other contractile machines, including T4 phage and R-type pyocins, provides a better understanding of how these systems have conserved similar functions/mechanisms despite evolution. We used the P. aeruginosa R2 pyocin as a structural template to build an atomic model of the TssB1C1 sheath in its extended conformation, allowing us to propose a coiled-spring-like mechanism for T6SS sheath contraction.

Type: Article
Title: Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.str.2017.12.005
Publisher version: http://doi.org/10.1016/j.str.2017.12.005
Language: English
Additional information: Copyright © 2017 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: T6SS, bacteriophage, cryo-EM, helical structure, molecular evolution
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10067065
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