Vandenberghe, ME;
Souedet, N;
Herard, A-S;
Ayral, A-M;
Letronne, F;
Balbastre, Y;
Sadouni, E;
... Delzescaux, T; + view all
(2018)
Voxel-Based Statistical Analysis o f 3D Immunostained Tissue Imaging.
Frontiers in Neuroscience
, 12
(754)
10.3389/fnins.2018.00754.
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Abstract
Recently developed techniques to visualize immunostained tissues in 3D and in large samples have expanded the scope of microscopic investigations at the level of the whole brain. Here, we propose to adapt voxel-based statistical analysis to 3D high-resolution images of the immunostained rodent brain. The proposed approach was first validated with a simulation dataset with known cluster locations. Then, it was applied to characterize the effect of ADAM30, a gene involved in the metabolism of the amyloid precursor protein, in a mouse model of Alzheimer's disease. This work introduces voxel-based analysis of 3D immunostained microscopic brain images and, therefore, opens the door to localized whole-brain exploratory investigation of pathological markers and cellular alterations.
| Type: | Article |
|---|---|
| Title: | Voxel-Based Statistical Analysis o f 3D Immunostained Tissue Imaging |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fnins.2018.00754 |
| Publisher version: | https://doi.org/10.3389/fnins.2018.00754 |
| Language: | English |
| Additional information: | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | voxel-based, 3D, immunohistochemistry, microscopy, rodent, Alzheimer’s disease |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Imaging Neuroscience |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10064000 |
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