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Variation in susceptibility to microbial lignin oxidation in a set of wheat straw cultivars: influence of genetic, seasonal and environmental factors

Konstantopoulou, M; Slator, PJ; Taylor, CR; Wellington, EM; Allison, G; Harper, AL; Bancroft, I; (2017) Variation in susceptibility to microbial lignin oxidation in a set of wheat straw cultivars: influence of genetic, seasonal and environmental factors. Nordic Pulp & Paper Research Journal , 32 (4) pp. 493-507. 10.3183/NPPRJ-2017-32-04-p493-507. Green open access

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Abstract

An alkali lignin preparation from wheat straw was found to be compatible with a previously developed nitrated lignin assay for microbial lignin oxidation, allowing comparison of the susceptibility of different wheat lignins to microbial oxidation. Alkali lignin was prepared from a panel of 18 wheat varieties, and was analysed by FT-IR spectroscopy, revealing minor variations in the lignin structure. Samples of nitrated alkali lignin were assayed against five lignin-degrading bacteria: Streptomyces viridosporus, Rhodococcus jostii RHA1, Pseudomonas putida mt-2, Microbacterium phyllosphaerae, and Sphingobacterium sp. T2. Up to 5-fold variation in rates of lignin oxidation were observed between different wheat varieties, and between different bacterial strain, with Sphingobacterium sp. T2 showing consistently highest absorbance changes. Testing of wheat varieties from two consecutive harvests, and from different locations, revealed that the observed variation was due to a combination of genetic, seasonal and environmental factors, but that some wheat varieties showed consistently higher rates of oxidation. Samples of wheat straw treated with Sphingobacterium sp. T2 were analysed by FT-IR spectroscopy after 7 and 14 days’ treatment, showing changes in lignin structure versus time, consistent with lignin breakdown.

Type: Article
Title: Variation in susceptibility to microbial lignin oxidation in a set of wheat straw cultivars: influence of genetic, seasonal and environmental factors
Open access status: An open access version is available from UCL Discovery
DOI: 10.3183/NPPRJ-2017-32-04-p493-507
Publisher version: http://dx.doi.org/10.3183/NPPRJ-2017-32-04-p493-50...
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Pseudomonas putida, Rhodococcus jostii RHA1, Sphingobacterium, Microbial lignin degradation, Wheat straw
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science
URI: https://discovery.ucl.ac.uk/id/eprint/10060155
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