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Stiffness memory of indirectly 3D-printed elastomer nanohybrid regulates, chondrogenesis and osteogenesis of human mesenchymal stem cells

Song, W; Wu, L; Magaz, A; Wang, T; Liu, C; Darbyshire, A; Loizidou, M; ... Birchall, M; + view all (2018) Stiffness memory of indirectly 3D-printed elastomer nanohybrid regulates, chondrogenesis and osteogenesis of human mesenchymal stem cells. Biomaterials , 186 pp. 64-79. 10.1016/j.biomaterials.2018.09.013. Green open access

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Abstract

The cellular microenvironment is dynamic, remodeling tissues lifelong. The biomechanical properties of the extracellular matrix (ECM) influence the function and differentiation of stem cells. While conventional artificial matrices or scaffolds for tissue engineering are primarily static models presenting well-defined stiffness, they lack the responsive changes required in dynamic physiological settings. Engineering scaffolds with varying elastic moduli is possible, but often lead to stiffening and chemical crosslinking of molecular structure with limited control over scaffold architecture. A family of indirectly 3D printed elastomeric nanohybrid scaffolds with thermoresponsive mechanical properties that soften by inverse self-assembling at body temperature have been developed recently. The initial stiffness and subsequent stiffness relaxation of the scaffolds regulated the proliferation and differentiation of human bone-marrow derived mesenchymal stem cells (hBM-MSCs) towards the chondrogenic and osteogenic lineages over 4 weeks, as measured by immunohistochemistry, histology, ELISA and qPCR. hBM-MSCs showed enhanced chondrogenic differentiation on softer scaffolds and osteogenic differentiation on stiffer ones, with similar relative expression to that of human femoral head tissue. Overall, stiffness relaxation favored osteogenic activity over chondrogenesis in vitro.

Type: Article
Title: Stiffness memory of indirectly 3D-printed elastomer nanohybrid regulates, chondrogenesis and osteogenesis of human mesenchymal stem cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.biomaterials.2018.09.013
Publisher version: http://doi.org/10.1016/j.biomaterials.2018.09.013
Language: English
Additional information: Copyright © The Author(s), 2018. This article is distributed under the terms of the Creative Commons License Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Keywords: Stiffness memory, stem cell differentiation, chondrogenesis, osteogenesis, 3D printing, elastomer nanohybrid
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > The Ear Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/10056895
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