Faure, LM;
Fiche, J-B;
Espinosa, L;
Ducret, A;
Anantharaman, V;
Luciano, J;
Lhospice, S;
... Mignot, T; + view all
(2016)
The mechanism of force transmission at bacterial focal adhesion complexes.
Nature
, 539
(7630)
pp. 530-535.
10.1038/nature20121.
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Abstract
Various rod-shaped bacteria mysteriously glide on surfaces in the absence of appendages such as flagella or pili. In the deltaproteobacterium Myxococcus xanthus, a putative gliding motility machinery (the Agl–Glt complex) localizes to so-called focal adhesion sites (FASs) that form stationary contact points with the underlying surface. Here we show that the Agl–Glt machinery contains an inner-membrane motor complex that moves intracellularly along a right-handed helical path; when the machinery becomes stationary at FASs, the motor complex powers a left-handed rotation of the cell around its long axis. At FASs, force transmission requires cyclic interactions between the molecular motor and the adhesion proteins of the outer membrane via a periplasmic interaction platform, which presumably involves contractile activity of motor components and possible interactions with peptidoglycan. Our results provide a molecular model of bacterial gliding motility.
| Type: | Article |
|---|---|
| Title: | The mechanism of force transmission at bacterial focal adhesion complexes |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/nature20121 |
| Publisher version: | https://doi.org/10.1542/10.1038/nature20121 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10055867 |
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