Miller, BS;
Parolo, C;
Turbé, V;
Keane, CE;
Gray, ER;
McKendry, R;
(2018)
Quantifying Biomolecular Binding Constants Using Video Paper Analytical Devices.
Chemistry – A European Journal
, 24
(39)
pp. 9783-9787.
10.1002/chem.201802394.
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Abstract
A novel ultra‐low‐cost biochemical analysis platform to quantify protein dissociation binding constants and kinetics using paper microfluidics is reported. This approach marries video imaging with one of humankind's oldest materials: paper, requiring no large, expensive laboratory equipment, complex microfluidics or external power. Temporal measurements of nanoparticle–antibody conjugates binding on paper is found to follow the Langmuir Adsorption Model. This is exploited to measure a series of antibody–antigen dissociation constants on paper, showing excellent agreement with a gold‐standard benchtop interferometer. The concept is demonstrated with a camera and low‐end smartphone, 500‐fold cheaper than the reference method, and can be multiplexed to measure ten reactions in parallel. These findings will help to widen access to quantitative analytical biochemistry, for diverse applications spanning disease diagnostics, drug discovery, and environmental analysis in resource‐limited settings.
| Type: | Article |
|---|---|
| Title: | Quantifying Biomolecular Binding Constants Using Video Paper Analytical Devices |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/chem.201802394 |
| Publisher version: | https://doi.org/10.1002/chem.201802394 |
| Language: | English |
| Additional information: | © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). |
| Keywords: | adsorption, nanoparticles, paper-based, protein-protein interaction, smartphones |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > MAPS Faculty Office |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10049310 |
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