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High-Density Protein Loading on Hierarchically Porous Layered Double Hydroxide Composites with a Rational Mesostructure

Tokudome, Y; Fukui, M; Tarutani, N; Nishimura, S; Prevot, V; Forano, C; Poologasundarampillai, G; ... Takahashi, M; + view all (2016) High-Density Protein Loading on Hierarchically Porous Layered Double Hydroxide Composites with a Rational Mesostructure. Langmuir , 32 (35) pp. 8826-8833. 10.1021/acs.langmuir.6b01925. Green open access

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Abstract

Hierarchically porous biocompatible Mg–Al–Cl-type layered double hydroxide (LDH) composites containing aluminum hydroxide (Alhy) have been prepared using a phase-separation process. The sol–gel synthesis allows for the hierarchical pores of the LDH–Alhy composites to be tuned, leading to a high specific solid surface area per unit volume available for high-molecular-weight protein adsorptions. A linear relationship between the effective surface area, SEFF, and loading capacity of a model protein, bovine serum albumin (BSA), is established following successful control of the structure of the LDH–Alhy composite. The threshold of the mean pore diameter, Dpm, above which BSA is effectively adsorbed on the surface of LDH–Alhy composites, is deduced as 20 nm. In particular, LDH–Alhy composite aerogels obtained via supercritical drying exhibit an extremely high capacity for protein loading (996 mg/g) as a result of a large mean mesopore diameter (>30 nm). The protein loading on LDH–Alhy is >14 times that of a reference LDH material (70 mg/g) prepared via a standard procedure. Importantly, BSA molecules pre-adsorbed on porous composites were successfully released on soaking in ionic solutions (HPO42– and Cl– aqueous). The superior capability of the biocompatible LDH materials for loading, encapsulation, and releasing large quantities of proteins was clearly demonstrated.

Type: Article
Title: High-Density Protein Loading on Hierarchically Porous Layered Double Hydroxide Composites with a Rational Mesostructure
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acs.langmuir.6b01925
Publisher version: http://dx.doi.org/10.1021/acs.langmuir.6b01925
Language: English
Additional information: This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Keywords: Science & Technology, Physical Sciences, Technology, Chemistry, Multidisciplinary, Chemistry, Physical, Materials Science, Multidisciplinary, Chemistry, Materials Science, Bovine Serum-Albumin, Adsorption Behavior, Nanoparticles, Separation, Immobilization, Microspheres, Monoliths, Chemistry, Scale
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10049117
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