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Quadrupole and hexadecapole transition dipole moment alignment in fluorescent protein Homo-FRET

Bain, AJ; MASTERS, T; (2018) Quadrupole and hexadecapole transition dipole moment alignment in fluorescent protein Homo-FRET. In: Andrews, David L. and Bain, Angus J. and Nunzi, Jean-Michel and Oste, Andreas, (eds.) Proceedings Nanophotonics VII; 106720Y (2018) - SPIE Photonics Europe, 2018. SPIE: Strasbourg, France. Green open access

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Abstract

Polarized time resolved fluorescence measurements are used to characterise the structure of the two-photon tensor in the enhanced green fluorescent protein (EGFP) and predict the “hidden” degree of hexadecapole transition dipole alignment 〈α40〉 created by two-photon absorption (TPA). We employ a new method for the accurate STED measurement of the evolution of 〈α40〉 by analysing the saturation dynamics of the orthogonally polarized components of two-photon excited EGFP fluorescence as a function of the time delay between the 800 nm pump and 570 nm dump pulses. The relaxation of 〈α40〉 by homo-FRET is found to be considerably greater than that for the fluorescence anisotropy which directly measures the quadrupolar transition dipole moment alignment 〈α20〉. Our results indicate that higher order dipole moment correlation measurements promise to be a sensitive probe of resonance energy transfer dynamics.

Type: Proceedings paper
Title: Quadrupole and hexadecapole transition dipole moment alignment in fluorescent protein Homo-FRET
Event: SPIE Photonics Europe, 2018 - Nanophotonics VII; 106720Y (2018)
Location: Strasbourg
Dates: 21 April 2018 - 26 April 2018
Open access status: An open access version is available from UCL Discovery
DOI: 10.1117/12.2306401
Publisher version: https://doi.org/10.1117/12.2306401
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Two-photon, molecular alignment, FRET, rotational diffusion, Stimulated Emission Depletion, STED, fluorescent protein
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy
URI: https://discovery.ucl.ac.uk/id/eprint/10048484
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