Burns, JR;
Lamarre, B;
Pyne, ALB;
Noble, JE;
Ryadnov, MG;
(2018)
DNA Origami Inside-Out Viruses.
ACS Synthetic Biology
, 7
(3)
pp. 767-773.
Preview |
Text
Pyne_et_al_revision.pdf - Accepted Version Download (892kB) | Preview |
Abstract
A synthetic topology for everted viruses is reported. The topology is a single-stranded virion DNA assembled into a hollow cube with exterior decorated with HIV-Tat transduction domains. The cube incorporates a pHresponsive lid allowing for the controlled encapsulation of functional proteins and their transfer and release into live cells. Unlike viruses, which are protein shells with a [3,5]-fold rotational symmetry that encase nucleic acids, these cubes are [3, 4]-fold DNA boxes encapsulating proteins. Like viruses, such everted DNA-built viruses are monodisperse nanoscale assemblies that infect human cells with a specialist cargo. The design offers a bespoke bottom-up platform for engineering nonpolyhedral, nonprotein synthetic viruses
| Type: | Article |
|---|---|
| Title: | DNA Origami Inside-Out Viruses |
| Open access status: | An open access version is available from UCL Discovery |
| Publisher version: | http://doi.org/10.1021/acssynbio.7600278 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | DNA origami; green fluorescent protein; microscopy; synthetic viruses; transfection |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10047309 |
Archive Staff Only
 |
View Item |
