Rosendahl, P;
Plak, K;
Jacobi, A;
Kraeter, M;
Toepfner, N;
Otto, O;
Herold, C;
... Guck, J; + view all
(2018)
Real-time fluorescence and deformability cytometry.
Nature Methods
, 15
pp. 355-358.
10.1038/nmeth.4639.
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Abstract
The throughput of cell mechanical characterization has recently approached that of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we developed an approach that combines real-time 1D-imaging fluorescence and deformability cytometry in one instrument (RT-FDC), thus opening many new research avenues. We demonstrated its utility by using subcellular fluorescence localization to identify mitotic cells and test for mechanical changes in those cells in an RNA interference screen.
| Type: | Article |
|---|---|
| Title: | Real-time fluorescence and deformability cytometry. |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/nmeth.4639 |
| Publisher version: | http://dx.doi.org/10.1038/nmeth.4639 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Biophysical methods, Flow cytometry, High-throughput screening, Lab-on-a-chip |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10047188 |
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