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Real-time fluorescence and deformability cytometry.

Rosendahl, P; Plak, K; Jacobi, A; Kraeter, M; Toepfner, N; Otto, O; Herold, C; ... Guck, J; + view all (2018) Real-time fluorescence and deformability cytometry. Nature Methods , 15 pp. 355-358. 10.1038/nmeth.4639. Green open access

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Abstract

The throughput of cell mechanical characterization has recently approached that of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we developed an approach that combines real-time 1D-imaging fluorescence and deformability cytometry in one instrument (RT-FDC), thus opening many new research avenues. We demonstrated its utility by using subcellular fluorescence localization to identify mitotic cells and test for mechanical changes in those cells in an RNA interference screen.

Type: Article
Title: Real-time fluorescence and deformability cytometry.
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/nmeth.4639
Publisher version: http://dx.doi.org/10.1038/nmeth.4639
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Biophysical methods, Flow cytometry, High-throughput screening, Lab-on-a-chip
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10047188
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