Passaro, D;
Abarrategi, A;
Foster, K;
Ariza-McNaughton, L;
Bonnet, D;
(2017)
Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging.
Journal of Visualized Experiments
, 126
, Article e55914. 10.3791/55914.
Preview |
Text
jove-protocol-55914-bioengineering-humanized-bone-marrow-microenvironments-mouse-their.pdf - Published Version Download (3MB) | Preview |
Abstract
Human hematopoietic stem cells (HSCs) reside in the bone marrow (BM) niche, an intricate, multifactorial network of components producing cytokines, growth factors, and extracellular matrix. The ability of HSCs to remain quiescent, self-renew or differentiate, and acquire mutations and become malignant depends upon the complex interactions they establish with different stromal components. To observe the crosstalk between human HSCs and the human BM niche in physiological and pathological conditions, we designed a protocol to ectopically model and image a humanized BM niche in immunodeficient mice. We show that the use of different cellular components allows for the formation of humanized structures and the opportunity to sustain long-term human hematopoietic engraftment. Using two-photon microscopy, we can live-image these structures in situ at the single-cell resolution, providing a powerful new tool for the functional characterization of the human BM microenvironment and its role in regulating normal and malignant hematopoiesis.
Type: | Article |
---|---|
Title: | Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging |
Location: | United States |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.3791/55914 |
Publisher version: | http://dx.doi.org/10.3791/55914 |
Language: | English |
Additional information: | Copyright © 2017 Creative Commons Attribution 3.0 License (https://creativecommons.org/licenses/by/3.0/). |
Keywords: | Live imaging, two-photon microscopy, bone marrow, stroma, microenvironment, humanized niche, human hematopoietic stem cells, human leukemia, vasculature, endothelial cells, mesenchymal stromal cells, BMP2 |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept |
URI: | https://discovery.ucl.ac.uk/id/eprint/10045375 |
Archive Staff Only
![]() |
View Item |