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Actin Waves Do Not Boost Neurite Outgrowth in the Early Stages of Neuron Maturation

Mortal, S; Iseppon, F; Perissinotto, A; D'Este, E; Cojoc, D; Napolitano, LMR; Torre, V; (2017) Actin Waves Do Not Boost Neurite Outgrowth in the Early Stages of Neuron Maturation. Frontiers in Cellular Neuroscience , 11 , Article 402. 10.3389/fncel.2017.00402. Green open access

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Abstract

During neurite development, Actin Waves (AWs) emerge at the neurite base and move up to its tip, causing a transient retraction of the Growth Cone (GC). Many studies have shown that AWs are linked to outbursts of neurite growth and, therefore, contribute to the fast elongation of the nascent axon. Using long term live cell-imaging, we show that AWs do not boost neurite outgrowth and that neurites without AWs can elongate for several hundred microns. Inhibition of Myosin II abolishes the transient GC retraction and strongly modifies the AWs morphology. Super-resolution nanoscopy shows that Myosin IIB shapes the growth cone-like AWs structure and is differently distributed in AWs and GCs. Interestingly, depletion of membrane cholesterol and inhibition of Rho GTPases decrease AWs frequency and velocity. Our results indicate that Myosin IIB, membrane tension, and small Rho GTPases are important players in the regulation of the AW dynamics. Finally, we suggest a role for AWs in maintaining the GCs active during environmental exploration.

Type: Article
Title: Actin Waves Do Not Boost Neurite Outgrowth in the Early Stages of Neuron Maturation
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fncel.2017.00402
Publisher version: http://dx.doi.org/10.3389/fncel.2017.00402
Language: English
Additional information: © 2017 Mortal, Iseppon, Perissinotto, D'Este, Cojoc, Napolitano and Torre. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Keywords: Science & Technology, Life Sciences & Biomedicine, Neurosciences, Neurosciences & Neurology, actin waves (AWs), growth cones (GCs), Myosin IIB, beta-cyclodextrin, RhoGTPases, MYOSIN-II, RHO-GTPASES, HIPPOCAMPAL AXONS, F-ACTIN, MOTILITY, MIGRATION, MEMBRANE, DYNAMICS, FAMILY, RAC
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Wolfson Inst for Biomedical Research
URI: https://discovery.ucl.ac.uk/id/eprint/10041376
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