Yeung, K; Boija, A; Karlsson, E; Holmqvist, P-H; Tstskis, Y; Nisoli, I; Yap, D; ... McNeill, H; + view all Yeung, K; Boija, A; Karlsson, E; Holmqvist, P-H; Tstskis, Y; Nisoli, I; Yap, D; Lorzadeh, A; Moksa, M; Hirst, M; Aparicio, S; Fanto, M; Stenberg, P; Mannervik, M; McNeill, H; - view fewer (2017) Atrophin controls developmental signaling pathways via interactions with Trithorax-like. eLIFE , 6 , Article e23084. 10.7554/eLife.23084.

Preview

Text Nisoli_elife-23084-v2.pdf - Published Version Download (9MB) | Preview

Abstract

Mutations in human Atrophin1, a transcriptional corepressor, cause dentatorubral-pallidoluysian atrophy, a neurodegenerative disease. Drosophila Atrophin (Atro) mutants display many phenotypes, including neurodegeneration, segmentation, patterning and planar polarity defects. Despite Atro’s critical role in development and disease, relatively little is known about Atro’s binding partners and downstream targets. We present the first genomic analysis of Atro using ChIP-seq against endogenous Atro. ChIP-seq identified 1300 potential direct targets of Atro including engrailed, and components of the Dpp and Notch signaling pathways. We show that Atro regulates Dpp and Notch signaling in larval imaginal discs, at least partially via regulation of thickveins and fringe. In addition, bioinformatics analyses, sequential ChIP and coimmunoprecipitation experiments reveal that Atro interacts with the Drosophila GAGA Factor, Trithorax-like (Trl), and they bind to the same loci simultaneously. Phenotypic analyses of Trl and Atro clones suggest that Atro is required to modulate the transcription activation by Trl in larval imaginal discs. Taken together, these data indicate that Atro is a major Trl cofactor that functions to moderate developmental gene transcription.

Download activity - last month

Export as JSONXMLCSV

Download activity - last 12 months

Export as JSONCSVXML

Downloads by country - last 12 months

Export as JSONXMLCSV

Archive Staff Only

View Item