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Multi-centre Raman spectral mapping of oesophageal cancer tissues: a study to assess system transferability

Isabelle, M; Dorney, J; Lewis, A; Lloyd, GR; Old, O; Shepherd, N; Rodriguez-Justo, M; ... Kendall, C; + view all (2016) Multi-centre Raman spectral mapping of oesophageal cancer tissues: a study to assess system transferability. Faraday Discussions , 187 pp. 87-103. 10.1039/c5fd00183h. Green open access

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Abstract

The potential for Raman spectroscopy to provide early and improved diagnosis on a wide range of tissue and biopsy samples in situ is well documented. The standard histopathology diagnostic methods of reviewing H&E and/or immunohistochemical (IHC) stained tissue sections provides valuable clinical information, but requires both logistics (review, analysis and interpretation by an expert) and costly processing and reagents. Vibrational spectroscopy offers a complimentary diagnostic tool providing specific and multiplexed information relating to molecular structure and composition, but is not yet used to a significant extent in a clinical setting. One of the challenges for clinical implementation is that each Raman spectrometer system will have different characteristics and therefore spectra are not readily compatible between systems. This is essential for clinical implementation where classification models are used to compare measured biochemical or tissue spectra against a library training dataset. In this study, we demonstrate the development and validation of a classification model to discriminate between adenocarcinoma (AC) and non-cancerous intraepithelial metaplasia (IM) oesophageal tissue samples, measured on three different Raman instruments across three different locations. Spectra were corrected using system transfer spectral correction algorithms including wavenumber shift (offset) correction, instrument response correction and baseline removal. The results from this study indicate that the combined correction methods do minimize the instrument and sample quality variations within and between the instrument sites. However, more tissue samples of varying pathology states and greater tissue area coverage (per sample) are needed to properly assess the ability of Raman spectroscopy and system transferability algorithms over multiple instrument sites.

Type: Article
Title: Multi-centre Raman spectral mapping of oesophageal cancer tissues: a study to assess system transferability
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/c5fd00183h
Publisher version: http://dx.doi.org/10.1039/c5fd00183h
Language: English
Additional information: Copyright © The Royal Society of Chemistry 2016.
Keywords: Science & Technology, Physical Sciences, Chemistry, Physical, Chemistry, Multiplicative Signal Correction, Barretts-esophagus, In-vitro, Objective Diagnosis, Ionizing-radiation, Calibration Model, Potential Tool, Breast-cancer, Lymph-nodes, Spectroscopy
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Pathology
URI: http://discovery.ucl.ac.uk/id/eprint/1482006
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