UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Comparison of the polyoma-virus early and late promoters by transcription in vitro

JAT, P; ROBERTS, JW; COWIE, A; KAMEN, R; (1982) Comparison of the polyoma-virus early and late promoters by transcription in vitro. Nucleic Acids Research , 10 (3) 871 - 887. 10.1093/nar/10.3.871. Green open access

[img]
Preview
PDF
Nucl._Acids_Res.-1982-Jat-871-87.pdf

Download (4MB)

Abstract

Polyoma virus DNA was transcribed in the HeLa whole cell extract in vitro system (1). Early region transcripts with the same 5′-ends as in vitro mRNAs, located 31±2bp from ‘TATA’-boxes, were synthesised by RNA polymerase II. Sequences sufficient for efficient expression of the early promoter were present in a substitution mutant lacking viral DNA from a position 55bp before the principal cap sites. Late region transcripts were synthesised inefficiently. Only one (at nt5129±2) of the many late mRNA cap sites functioned as an in vitro initiation point. This was the one 5′-end located 31±2bp from a sequence resembling the ‘TATA’ consensus. The proportion of late to early region RNA polymerase II transcripts decreased dramatically at suboptimal template concentrations. An hypothesis to explain the regulation of late gene expression in vivo based on these results is proposed. Although linear templates were transcribed only by RNA polymerase II, transcripts with the same sense as late mRNAs and 5′-ends at nt5076±2 were produced from superhelical templates by an α-amanitin resistant enzyme.

Type: Article
Title: Comparison of the polyoma-virus early and late promoters by transcription in vitro
Open access status: An open access version is available from UCL Discovery
DOI: 10.1093/nar/10.3.871
Publisher version: http://dx.doi.org/10.1093/nar/10.3.871
Language: English
Additional information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Institute of Prion Diseases
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Institute of Prion Diseases > MRC Prion Unit at UCL
URI: http://discovery.ucl.ac.uk/id/eprint/143260
Downloads since deposit
243Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item