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Functional imaging in microfluidic chambers reveal sensory neuron sensitivity is differentially regulated between neuronal regions

Clark, AJ; Menendez, G; AlQatari, M; Patel, N; Arstad, E; Schiavo, G; Koltzenburg, M; (2018) Functional imaging in microfluidic chambers reveal sensory neuron sensitivity is differentially regulated between neuronal regions. PAIN , 159 (7) pp. 1413-1425. 10.1097/j.pain.0000000000001145. Green open access

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Abstract

Primary afferent sensory neurons are incredibly long cells, often traversing distances of over one metre in humans. Cutaneous sensory stimuli are transduced in the periphery by specialised endorgans or free nerve endings which code the stimulus into electrical action potentials that propagate towards the central nervous system. Despite significant advances in our knowledge of sensory neuron physiology and ion channel expression, many commonly used techniques fail to accurately model the primary afferent neuron in its entirety. In vitro experiments often focus on the cell somata and neglect the fundamental processes of peripheral stimulus transduction and action potential propagation. Despite this, these experiments are commonly used as a model for cellular investigations of the receptive terminals. We demonstrate that ratiometric calcium imaging performed in compartmentalised sensory neuron cultures can be used to directly and accurately compare the sensitivity and functional protein expression of isolated neuronal regions in vitro. Using microfluidic chambers, we demonstrate that the nerve terminals of cultured DRG neurons can be depolarised to induce action potential propagation, which has both TTX-resistant and TTXsensitive components. Furthermore, we show that there is a differential regulation of proton sensitivity between the sensory terminals and somata in cultured sensory neurons. We also demonstrate that capsaicin sensitivity is highly dependent on embryonic dissection age. This approach enables a comprehensive method to study the excitability and regional sensitivity of cultured sensory neurons on a single cell level. Examination of the sensory terminals is crucial to further understand the properties and diversity of DRG sensory neurons.

Type: Article
Title: Functional imaging in microfluidic chambers reveal sensory neuron sensitivity is differentially regulated between neuronal regions
Open access status: An open access version is available from UCL Discovery
DOI: 10.1097/j.pain.0000000000001145
Publisher version: http://dx.doi.org/10.1097/j.pain.0000000000001145
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Sodium channels, Lidocaine, Tetrodotoxin, Capsaicin, Development, TRPV1, ASIC, NGF, GDNF
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Metabolism and Experi Therapeutics
URI: http://discovery.ucl.ac.uk/id/eprint/10024687
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