Soueid, A; Hall, S; Mudera, V; (2005) Profile of pulmonary smooth muscle cells and their response to blockade and Stimulations. In: European Cells and Materials. (pp. 19 - 19).

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Abstract

INTRODUCTION: The tone of the pulmonary arteries is the summation of the activity of each smooth muscle cell (SMC) within a vessel wall and its interaction with the endothelial cells and extracellular matrix (including collagen). There are reported phenotypic differences between SMC in the inner & outer layers of pulmonary artery walls1. To tissue engineer a blood vessel a basic understanding of differences between inner and outer SMC’s in terms of attachment and contraction of 3D matrix is essential. We hypothesised that they will differ in their ability to contract a 3D collagen gel. Using a Culture Force Monitor (CFM) we sought to: (1) quantify the contractile ability of SMC derived from inner and outer normal and hypoxic arteries, harvested from piglet models, over 24 hours, (2) test the effect of cytoskeletal blockade on force retention in collagen gels and (3) quantify cellular contractile response to agonist or antagonist stimulation.

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