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Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans

Unkruer, B; Pekcec, A; Fuest, C; Wehmeyer, A; Balda, MS; Horn, A; Baumgartner, W; (2009) Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans. BMC Neuroscience , 10 , Article 28. 10.1186/1471-2202-10-28. Green open access

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Abstract

Background: The Y-box binding protein 1 (YB-1) is considered to be one of the key regulators of transcription and translation. However, so far only limited knowledge exists regarding its cellular distribution in the adult brain.Results: Analysis of YB-1 immunolabelling as well as double-labelling with the neuronal marker NeuN in rat brain tissue revealed a predominant neuronal expression in the dentate gyrus, the cornu ammonis pyramidal cell layer, layer III of the piriform cortex as well as throughout all layers of the parahippocampal cortex. In the hilus of the hippocampus single neurons expressed YB-1. The neuronal expression pattern was comparable in the hippocampus and parahippocampal cortex of adult macaques and humans. Double-labelling of YB-1 with the endothelial cell marker Glut-1, the multidrug transporter P-glycoprotein, and the astrocytic marker GFAP did not indicate a co-localization. Following status epilepticus in rats, no induction of YB-1 occurred in brain capillary endothelial cells and neurons.Conclusion: In conclusion, our study demonstrates that YB-1 is predominantly expressed in neurons in the adult brain of rats, macaques and humans. Lack of a co-localization with Glut-1 and P-glycoprotein argues against a direct role of YB-1 in the regulation of blood-brain barrier P-glycoprotein.

Type: Article
Title: Cellular localization of Y-box binding protein 1 in brain tissue of rats, macaques, and humans
Open access status: An open access version is available from UCL Discovery
DOI: 10.1186/1471-2202-10-28
Publisher version: http://dx.doi.org/10.1186/1471-2202-10-28
Language: English
Additional information: © 2009 Unkrüer et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: P-GLYCOPROTEIN EXPRESSION, TRANSCRIPTION FACTOR YB-1, NUCLEAR EXPRESSION, DRUG-RESISTANCE, BREAST-CANCER, ABC TRANSPORTERS, GENE-EXPRESSION, UP-REGULATION, CANINE BRAIN, CHEMOTHERAPY
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/91229
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