UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Cloning and functional-analysis of the tata-binding protein from Sulfolobus-shibatae

QURESHI, SA; BAUMANN, P; ROWLANDS, T; KHOO, B; JACKSON, SP; (1995) Cloning and functional-analysis of the tata-binding protein from Sulfolobus-shibatae. Nucleic Acids Research , 23 (10) 1775 - 1781. Green open access

[img]
Preview
PDF
Nucleic_Acids_Res_1995_Qureshi.pdf

Download (1MB)

Abstract

Archaea (formerly archaebacteria) comprise a domain of life that is phylogenetically distinct from both Eucarya and Bacteria. Here we report the cloning of a gene from the Archaeon Sulfolobus shibatae that encodes a protein with strong homology to the TATA binding protein (TBP) of eukaryotes. Sulfolobus shibatae TBP is, however, almost as diverged from other archaeal TBPs that have been cloned as it is from eukaryotic TBPs. DNA binding studies indicate that S.shibatae TBP recognizes TATA-like A-box sequences that are present upstream of most archaeal genes. By quantitatively immunodepleting S.shibatae TBP from an in vitro transcription system, we demonstrate that Sulfolobus RNA polymerase is capable of transcribing the 16S/23S rRNA promoter weakly in the absence of TBP. Most significantly, we show that addition of recombinant S.shibatae TBP to this immunodepleted system leads to transcriptional stimulation and that this stimulation is dependent on the A-box sequence of the promoter. Taken together, these findings reveal fundamental similarities between the transcription machineries of Archaea and eukaryotes.

Type: Article
Title: Cloning and functional-analysis of the tata-binding protein from Sulfolobus-shibatae
Open access status: An open access version is available from UCL Discovery
Publisher version: http://nar.oxfordjournals.org/
Language: English
Additional information: Nucleic Acids Research is an Open Access Journal. © 2005 Oxford University Press
Keywords: EUKARYOTIC TRANSCRIPTION FACTOR, CELL-FREE TRANSCRIPTION, RNA-POLYMERASE, METHANOCOCCUS-VANNIELII, ARCHAEBACTERIAL PROMOTER, NUCLEOTIDE-SEQUENCE, FACTOR-IIIB, FACTOR-IIB, GENES, BOX
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Experimental and Translational Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/90804
Downloads since deposit
76Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item