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Emergence of functional sensory subtypes as defined by transient receptor potential channel expression

Hjerling-Leffler, J; AlQatari, M; Ernfors, P; Koltzenburg, M; (2007) Emergence of functional sensory subtypes as defined by transient receptor potential channel expression. J NEUROSCI , 27 (10) 2435 - 2443. 10.1523/JNEUROSCI.5614-06.2007. Green open access

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Abstract

The existence of heterogeneous populations of dorsal root ganglion (DRG) neurons conveying different somatosensory information is the basis for the perception of touch, temperature, and pain. A differential expression of transient receptor potential (TRP) cation channels contributes to this functional heterogeneity. However, little is known about the development of functionally diverse neuronal subpopulations. Here, we use calcium imaging of acutely dissociated mouse sensory neurons and quantitative reverse transcription PCR to show that TRP cation channels emerge in waves, with the diversification of functional groups starting at embryonic day 12.5 (E12.5) and extending well into the postnatal life. Functional responses of voltage-gated calcium channels were present in DRG neurons at E11.5 and reached adult levels by E14.5. Responses to capsaicin, menthol, and cinnamaldehyde were first seen at E12.5, E16.5, and postnatal day 0 (P0), when the mRNA for TRP cation channel, subfamily V, member 1 (TRPV1), TRP cation channel, subfamily M, member 8 (TRPM8), and TRP cation channel, subfamily A, member 1 (TRPA1), respectively, was first detected. Cold-sensitive neurons were present before the expression or functional responses of TRPM8 or TRPA1. Our data support a lineage relationship in which TRPM8- and TRPA1-expressing sensory neurons derive from the population of TRPV1-expressing neurons. The TRPA1 subpopulation of neurons emerges independently in two distinct classes of nociceptors: around birth in the peptidergic population and after P14 in the nonpeptidergic class. This indicates that neurons with similar receptive properties can be generated in different sublineages at different developmental stages. This study describes for the first time the emergence of functional subtypes of sensory neurons, providing new insight into the development of nociception and thermoreception.

Type: Article
Title: Emergence of functional sensory subtypes as defined by transient receptor potential channel expression
Open access status: An open access version is available from UCL Discovery
DOI: 10.1523/JNEUROSCI.5614-06.2007
Keywords: TRP cation channel, functional development, dorsal root ganglion, lineage relationship, mouse, sensory neuron, ROOT GANGLION NEURONS, NERVE GROWTH-FACTOR, NOCICEPTIVE NEURONS, CAPSAICIN RECEPTOR, ION-CHANNEL, NEUROTROPHIC FACTOR, TRP CHANNELS, C-FIBERS, COLD, SURVIVAL
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences
URI: https://discovery.ucl.ac.uk/id/eprint/8532
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