UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Functional interaction between the ZO-1-interacting transcription factor ZONAB/DbpA and the RNA processing factor symplekin

Kavanagh, E; Buchert, M; Tsapara, A; Choquet, A; Balda, MS; Hollande, F; Matter, K; (2006) Functional interaction between the ZO-1-interacting transcription factor ZONAB/DbpA and the RNA processing factor symplekin. J CELL SCI , 119 (24) 5098 - 5105. 10.1242/jcs.03297. Green open access

[img]
Preview
PDF
8238.pdf

Download (1MB)

Abstract

Epithelial tight junctions participate in the regulation of gene expression by controlling the activity of transcription factors that can interact with junctional components. One such protein is the Y-box transcription factor ZONAB/DbpA that binds to ZO-1, a component of the junctional plaque. Symplekin, another nuclear protein that can associate with tight junctions, functions in the regulation of polyadenylation and thereby promotes gene expression. Here, we addressed the question of whether these two proteins interact and whether this is of functional relevance. We demonstrate that ZONAB/DbpA and symplekin form a complex in kidney and intestinal epithelial cells that can be immunoprecipitated and that exists in the nucleus. The interaction between ZONAB/DbpA and symplekin can be reconstituted with recombinant proteins. In reporter gene assays in which ZONAB/DbpA functions as a repressor, symplekin functionally interacts with ZONAB/DbpA, indicating that symplekin can also promote transcriptional repression. RNAi experiments indicate that symplekin depletion reduces the nuclear accumulation and the transcriptional activity of ZONAB/DbpA in colon adenocarcinoma cells, resulting in inhibition of proliferation and reduced expression of the ZONAB/DbpA-target gene cyclin D1. Our data thus indicate that symplekin and ZONAB/DbpA cooperate in the regulation of transcription, and that they promote epithelial proliferation and cyclin D1 expression.

Type: Article
Title: Functional interaction between the ZO-1-interacting transcription factor ZONAB/DbpA and the RNA processing factor symplekin
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/jcs.03297
Keywords: Y-box factor, erbB-2, adenocarcinoma, cyclin D1, cell cycle, epithelia, JUNCTION PROTEIN ZO-1, ACID-BINDING PROTEIN, TIGHT JUNCTION, ZONULA OCCLUDENS-1, PARACELLULAR PERMEABILITY, CELL-ADHESION, GAP-JUNCTIONS, MOUSE-BRAIN, PDZ DOMAIN, EXPRESSION
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/8238
Downloads since deposit
391Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item