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Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

Huggett, JF; Novak, T; Garson, JA; Green, C; Morris-Jones, SD; Miller, RF; Zumla, A; (2008) Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon. BMC Research Notes , 1 , Article 70. 10.1186/1756-0500-1-70. Green open access

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Abstract

PCR inhibition by nucleic acid extracts is a well known yet poorly described phenomenon. Inhibition assessment generally depends on the assumption that inhibitors affect all PCR reactions to the same extent; i.e. that the reaction of interest and the control reaction are equally susceptible to inhibition. To test this assumption we performed inhibition assessment on DNA extracts from human urine samples, fresh urine and EDTA using different PCR reactions.

Type: Article
Title: Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon
Location: England
Identifier: PMCID: PMC2564953
Open access status: An open access version is available from UCL Discovery
DOI: 10.1186/1756-0500-1-70
Publisher version: http://dx.doi.org/10.1186/1756-0500-1-70
Language: English
Additional information: © 2008 Huggett et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute for Global Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute for Global Health > Infection and Population Health
URI: https://discovery.ucl.ac.uk/id/eprint/80337
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