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Protein engineering as a tool for crystallography

Price, SR; Nagai, K; (1995) Protein engineering as a tool for crystallography. Current Opinion in Biotechnology , 6 (4) 425 - 430. 10.1016/0958-1669(95)80072-7. Green open access

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Abstract

The generation of large quantities of protein by overexpression technology has enabled structural studies of many important molecules that are found in only minute quantities in the cell. An increasing number of structures of proteins overexpressed in non-native systems have been solved. Crystallographers now have an extremely powerful tool, namely protein engineering, for the generation of native and derivative crystals that diffract to high solution. The mutation of residues or generation of compact domains through truncation has resulted in crystals with enhanced diffraction properties. Heavy atom derivative crystals isomorphous to the native protein may also be engineered either by introducing cysteines or by removing cysteines whose reaction with heavy-atom compounds results in poor crystals.

Type: Article
Title: Protein engineering as a tool for crystallography
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/0958-1669(95)80072-7
Publisher version: http://dx.doi.org/10.1016/0958-1669(95)80072-7
Language: English
Additional information: This article was published in Current Opinion in Biotechnology, 6(4), Stephen R. Price, Kiyoshi Nagai, Protein engineering as a tool for crystallography, pp.425-430, Copyright Elsevier 1995. Permission is granted to post the published paper as per the discretion of the Publishers. The Current Opinion in Biology journal homepage can be found here: http://www.sciencedirect.com/science/journal/09581669.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/76347
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