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Genomic homogeneity in fibrolamellar carcinomas

Sirivatanauksorn, Y; Sirivatanauksorn, V; Lemoine, NR; Williamson, RCN; Davidson, BR; (2001) Genomic homogeneity in fibrolamellar carcinomas. GUT , 49 (1) 82 - 86. Green open access

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Abstract

Background-Fibrolamellar carcinoma (FLC) is a variant of hepatocellular carcinoma (HCC) with distinctive clinical and histological features. To date there have been few studies on the genotypic aspects of FLC and no previous attempts have been made to use the arbitrarily primed-polymerase chain reaction (AF-FCR) technique to detect genetic alterations in this disease.Aim-The aim of this study was to assess the degree of genomic heterogeneity of FEC using the AP-PCR technique. Methods-A fetal of 50 tissue samples of primary and metastatic FLCs from seven patients were microdissected. AP-PCR amplification of each genomic DNA sample was carried out using two arbitrary primers.Results-DNA fingerprints of the primary FLCs and all their metastatic lesions (both synchronous and metachronous disease) were identical in an individual patient. The fingerprints were different between tumours of different patients. No evidence of intratumour heterogeneity was observed.Conclusions-Such genomic homogeneity in FLCs may explain their indolent growth. The absence of clonal evolution, which is present in other tumours (particularly HCCs), may explain the distinct behaviour in this tumour. The tumorigenic pathway and degree of somatic genomic changes in this disease may be less complex than in HCC.

Type: Article
Title: Genomic homogeneity in fibrolamellar carcinomas
Open access status: An open access version is available from UCL Discovery
Keywords: fibrolamellar carcinoma, hepatocellular carcinoma, DNA fingerprint, arbitrarily primed-polymerase chain reaction, laser capture microdissection, POLYMERASE-CHAIN-REACTION, HEPATOCELLULAR-CARCINOMA, ALLELIC LOSSES, LIVER, PCR, TRANSPLANTATION, HETEROGENEITY, HEPATECTOMY, HEPATOMA, PRIMERS
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/7563
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