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Validation and clinical application of molecular methods for the identification of molds in tissue

Paterson, PJ; Seaton, S; McHugh, TD; McLaughlin, J; Potter, M; Prentice, HG; Kibbler, CC; (2006) Validation and clinical application of molecular methods for the identification of molds in tissue. CLIN INFECT DIS , 42 (1) 51 - 56. Green open access

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Abstract

Background. Invasive fungal infections due to less-common molds are an increasing problem, and accurate diagnosis is difficult.Methods. We used our previously established molecular method, which allows species identification of molds in histological tissue sections, to test sequential specimens from 56 patients with invasive fungal infections who were treated at our institution from 1982 to 2000.Results. The validity of the method was demonstrated with the establishment of a molecular diagnosis in 52 cases (93%). Confirmation of the causative organism was made in all cases in which a mold had been cultured from the tissue specimen. Less-common molds were identified in 7% of cases and appear to be an increasing problem.Conclusions. Our previously established method has proven to be of value in determining the incidence of invasive infection caused by less-common molds. Institutions should continue to pursue diagnosis of invasive fungal infections by means of tissue culture and microbiologic analysis.

Type: Article
Title: Validation and clinical application of molecular methods for the identification of molds in tissue
Open access status: An open access version is available from UCL Discovery
Keywords: INVASIVE PULMONARY ASPERGILLOSIS, IN-SITU HYBRIDIZATION, FUNGAL PATHOGENS, RIBOSOMAL-RNA, INFECTIONS, MANAGEMENT, DIAGNOSIS, SECTIONS, PROBES
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/7096
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