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Lack of effect of the human GM-CSF analog E21R on the survival of primary human acute myeloid leukemia cells

Jakupovic, I.; Grandage, V.L.; Linch, D.C.; Khwaja, A.; (2004) Lack of effect of the human GM-CSF analog E21R on the survival of primary human acute myeloid leukemia cells. Blood , 103 (8) pp. 3230-3232. 10.1182/blood-2003-06-1985. Green open access

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Abstract

The granulocyte-macrophage colony-stimulating factor (GM-CSF) analog E21R binds to the GM-CSF receptor complex with low affinity and acts as a competitive antagonist. In addition, it has been reported to be a potent direct activator of apoptosis in primary human acute myeloid leukemia (AML) cells. We have confirmed the ability of E21R to neutralize the biologic effects of GM-CSF and investigated its activity on primary AML blasts. We find that it failed to induce cell death in blast cells from 23 separate AML cases treated in vitro at concentrations of E21R up to 30 µg/mL. Significant cell death resulted in all cases after incubation with cytosine arabinoside. The lack of effect of E21R on AML blasts was unlikely to be due to an absence of functional GM-CSF receptors because 13 cases demonstrated an increase in cell number with the addition of exogenous GM-CSF. These results do not support the use of E21R for the treatment of AML.

Type: Article
Title: Lack of effect of the human GM-CSF analog E21R on the survival of primary human acute myeloid leukemia cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1182/blood-2003-06-1985
Publisher version: http://dx.doi.org/10.1182/blood-2003-06-1985
Language: English
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
URI: https://discovery.ucl.ac.uk/id/eprint/6908
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