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A doublecortin containing microtubule-associated protein is implicated in mechanotransduction in Drosophila sensory cilia

Bechstedt, S; Albert, JT; Kreil, DP; Muller-Reichert, T; Gopfert, MC; Howard, J; (2010) A doublecortin containing microtubule-associated protein is implicated in mechanotransduction in Drosophila sensory cilia. Nature Communications , 1 , Article 11. 10.1038/ncomms1007. Green open access

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Abstract

Mechanoreceptors are sensory cells that transduce mechanical stimuli into electrical signals and mediate the perception of sound, touch and acceleration. Ciliated mechanoreceptors possess an elaborate microtubule cytoskeleton that facilitates the coupling of external forces to the transduction apparatus. In a screen for genes preferentially expressed in Drosophila campaniform mechanoreceptors, we identified DCX-EMAP, a unique member of the EMAP family (echinoderm-microtubule-associated proteins) that contains two doublecortin domains. DCX-EMAP localizes to the tubular body in campaniform receptors and to the ciliary dilation in chordotonal mechanoreceptors in Johnston's organ, the fly's auditory organ. Adult flies carrying a piggyBac insertion in the DCX-EMAP gene are uncoordinated and deaf and display loss of mechanosensory transduction and amplification. Electron microscopy of mutant sensilla reveals loss of electron-dense materials within the microtubule cytoskeleton in the tubular body and ciliary dilation. Our results establish a catalogue of candidate genes for Drosophila mechanosensation and show that one candidate, DCX-EMAP, is likely to be required for mechanosensory transduction and amplification.

Type: Article
Title: A doublecortin containing microtubule-associated protein is implicated in mechanotransduction in Drosophila sensory cilia
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/ncomms1007
Publisher version: http://dx.doi.org/10.1038/ncomms1007
Language: English
Additional information: This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
Keywords: mechanosensory transduction, C-elegans, caenorhabditis-elegans, genetic dissection, binding protein, fine-structure, TRP channels, WD-repeat, EMAP, melanogaster
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > The Ear Institute
URI: https://discovery.ucl.ac.uk/id/eprint/408975
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