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The pharmacology of hSK1 Ca2+-activated K+ channels expressed in mammalian cell lines.

Shah, M; Haylett, DG; (2000) The pharmacology of hSK1 Ca2+-activated K+ channels expressed in mammalian cell lines. Br J Pharmacol , 129 (4) 627 - 630. 10.1038/sj.bjp.0703111. Green open access

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Abstract

The pharmacology of hSK1, a small conductance calcium-activated potassium channel, was studied in mammalian cell lines (HEK293 and COS-7). In these cell types, hSK1 forms an apamin-sensitive channel with an IC(50) for apamin of 8 nM in HEK293 cells and 12 nM in COS-7 cells. The currents in HEK293 cells were also sensitive to tubocurarine (IC(50)=23 microM), dequalinium (IC(50)=0.4 microM), and the novel dequalinium analogue, UCL1848 (IC(50)=1 nM). These results are very different from the pharmacology of hSK1 channels expressed in Xenopus oocytes and suggest the properties of the channel may depend on the expression system. Our findings also raise questions about the role of SK1 channels in generating the apamin-insensitive slow afterhyperpolarization observed in central neurones.

Type: Article
Title: The pharmacology of hSK1 Ca2+-activated K+ channels expressed in mammalian cell lines.
Location: ENGLAND
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/sj.bjp.0703111
Publisher version: http://dx.doi.org/10.1038/sj.bjp.0703111
Language: English
Additional information: PMCID: PMC1571896
Keywords: Animals, Apamin, COS Cells, Calcium, Cell Line, Cercopithecus aethiops, Dequalinium, Humans, Kidney, Nicotinic Antagonists, Potassium Channel Blockers, Potassium Channels, Potassium Channels, Calcium-Activated, Rats, Small-Conductance Calcium-Activated Potassium Channels, Transfection, Tubocurarine, Xenopus
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/38984
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