UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Combining confocal and BSE SEM imaging for bone block surfaces

Boyde, A; Lovicar, L; Zamecnik, J; (2005) Combining confocal and BSE SEM imaging for bone block surfaces. European Cells and Materials , 9 33 - 38. Green open access

[thumbnail of v009a05.pdf]
Preview
PDF
v009a05.pdf
Available under License : See the attached licence file.

Download (166kB)

Abstract

The present report presents a method for the correlation of qualitative and quantitative BSE SEM imaging with confocal scanning light microscopy (CSLM) imaging modes applied to bone samples embedded in PMMA. The SEM has a proper digital scan generator: we leave the BSE image unchanged, and match the CSLM image to it, because the CSLM scan mechanism is not digital, though the signal is digitised. Our overlapping program uses a linear transformation matrix which projects one system to the other, calculated by finding three corresponding points in BSE and CSLM pictures. BSE images are empty where cells and osteoid are present. Fluorescence mode CSLM fills in these gaps. The combination images enhance our understanding of what is going on - and re-establish the need for good cellular preservation.

Type: Article
Title: Combining confocal and BSE SEM imaging for bone block surfaces
Open access status: An open access version is available from UCL Discovery
Publisher version: http://www.ecmjournal.org/journal/papers/vol009/vo...
Language: English
Additional information: Reproduced with kind permission from eCM journal (www.ecmjournal.org)
Keywords: Bone, scanning electron microscopy, confocal optical microscopy, block face microscopy, osteoid, osteoblasts, osteocytes
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
URI: https://discovery.ucl.ac.uk/id/eprint/23776
Downloads since deposit
389Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item